楊梅素 Myricetin主要功能：

1）抗氧化的作用：楊梅素 Myricetin是一種很強的抗氧化劑，氧化應激在各種神經疾病包括局部缺血和老年期癡呆中起關鍵作用。楊梅素通過構象變化作用減少β-澱粉酶的產生和毒性，可用於統計老年癡呆的進展狀況。

2）抗腫瘤作用：楊梅素 Myricetin是一種有效的致癌作用化學防治劑。

3）降低神經毒性：楊梅素 Myricetin可以通過不同途徑抑制由谷氨酸引起的神經毒性保護神經元，從而有效阻止神經損傷。

4）對淋巴細胞活化及增殖的影響：楊梅素 Myricetin能抑制T細胞早期活化指標CD69的表達，並能抑制淋巴細胞增殖反應。

5）血小板活化因子（PAF）的拮抗作用：楊梅素體外呈濃度依賴性地抑制PAF誘發的WRP聚集及52HT釋放。同時能明顯抑制PAF引起的血小板內游離鈣的增高。因此楊梅素具有抗血栓、抗心肌缺血、改善微循環等多方面的心血管藥理作用。

6）降血糖作用：楊梅素 Myricetin具有較為明顯的降血糖作用。

7）保肝護肝的作用：楊梅素能明顯抑制血清中丙氨酸轉氨酶（ALT）和天冬氨酶轉氨酶（AST）的活性升高及降低血清總膽紅素，有明顯的降酶退黃的作用。

標 題：HPLC法測定二氫楊梅素含量的操作規程

起草單位：長沙上禾生物科技有限公司

目  的：HPLC法測定二氫楊梅素含量的操作規程

責任人：QC員。

內  容：

1、 試劑

1.1 甲醇（HPLC級）

1.2 蒸餾水（AR級）

1.3冰醋酸（HPLC級）

2、 對照品

二氫楊梅素（購自中國藥品生物制品檢定所）

3、 儀器和用具

3.1 電子天平（1/100000）

3.2 玻璃儀器：100ml容量瓶

3.3 安捷倫1220 HPLC

4、液相條件

4.1流動相：甲醇：水：冰醋酸=50:50:1

4.2檢測波長：292nm

4.3流速：1ml/min

4.4柱溫：30℃

5 、  操作步驟

5.1 對照品溶液的制備  精密稱取在110℃乾燥至恆重的二氫楊梅素對照品10mg，置50ml容量瓶中，加50%甲醇適量，超聲處理10min使溶解，放冷，加50%甲醇至刻度，搖勻，備用。

 5.2樣品溶液制備  精密稱取在110℃乾燥至恆重的二氫楊梅素樣品10mg，置50ml容量瓶中，加50%甲醇適量，超聲處理10min使溶解，放冷，加50%甲醇至刻度，搖勻，備用。

6、檢測

取對照品溶液和樣品溶液0.22μm針孔過濾器過濾，進樣20µl，根據峰面積計算樣品含量。

含量按以下公式計算：

         A2 x C1 x T

  R=----------------- x 100

    A1 x C2

C1: 對照品的參考濃度，mg/ml

C2: 樣品的參考濃度，mg/ml

A1: 對照品溶液的色譜主峰值面積

A2: 樣品溶液的色譜主峰值面積

T:  對照品的含量

 

參考文獻：

1.A. Umarov, V.S. Batyk, A.M. Khaleckii, Chem. Nat. Comp. 7, 499 (1971)

2.V.A. Soboleva, R.K. Chagovets, Chem. Nat. Comp. 7, 509 (1971)

3.R.K. Chagovets, I.M. Persev, V.O. Soboleva, Farm. Zh. 22, 64 (1967)

4.O.M. Bondarenko, R.K. Chagovets, V.I. Litvinenko, G.V. Obolensova, B.I. Sila, T.B. Kigel, Farm. Zh. 26, 46 (1971)

5.K. Miller, R. Pohl, Planta Med. 18, 114 (1970)

6.V.A. Soboleva, R.K. Chagovets, O.I. Gladkaya, //In proceeding: Tez 2-go simpoziuma po fenolnim soedineniyam., (Alma-Ata, 1970), p. 148

 7.Effects of myricetin on the bioavailability of doxorubicin for oral drug delivery in Rats: Possible role of CYP3A4 and P-glycoprotein inhibition by myricetin
The purpose of this study was to investigate the effect of oral myricetin on the bioavailability and pharmacokinetics of orally ... rats for oral delivery. The effect of myricetin on the P-glycoprotein (P-gp) and...

Sang-Joon Choi, Sang-Chul Shin, Jun-Shik Choi in Archives of Pharmacal Research (2011)

8.A glycoside of myricetin from the flowers of Hibiscus cannabinusZ. P. Pakudina, B. Maksudova in Chemistry of Natural Compounds (1976)

Für die elektrophoretische Auftrennung und densitometrische Bestimmung der Flavonoide Kämpferol (1), Quercetin (2), Quercetagerol (3), Myricetin (4) und der Heteroside Robinosid (5), Quercitrosid (6), Isoquercitrosid (7), Rutosid (8) und Myricitrosid (9)
K. Söllner in Fresenius' Zeitschrift für analytische Chemie (1960)

9.Evaluation of the antioxidant potential of myricetin 3-O-α-L-rhamnopyranoside and myricetin 4′-O-α-L-rhamnopyranoside through a computational study
In this work, we present a computational study on the antioxidant potential of myricetin 3-O-α-L-rhamnopyranoside (Compound M3) and myricetin 4′-O-α-L-rhamnopyranoside (Compound M4′). Structural parameters, bond ...
Rodrigo A. Mendes, Shawan K. C. Almeida, Iuri N. Soares… in Journal of Molecular Modeling (2019)

10.Spektrophotometrische und fluorometrische Bestimmung von Myricetin (3,3′,4′,5,5′,7-Hexahydroxy-Flavon)
Myricetin (3,3′,4′,5,5′,...−4% Myricetin lassen sich mit einem relativen Fehler von...
Antonia S. Zaprjanowa in Microchimica Acta (1976)

11.Analgesic activity of myricetin isolated from Myrica rubra Sieb. et Zucc. leaves
Myrica rubra Sieb. et Zucc....leaves are commonly used as an astringent, antidiarrheic, and analgesics in folk medicine in China. In the present study, the analgesic activity of myricetin

Yan Tong, Xiao-Mian Zhou, Shu-Jun Wang, Yang Yang… in Archives of Pharmacal Research (2009)

12.Inhibition of interleukin-12 production in mouse macrophagesvia decreased nuclear factor-κB DNA binding activity by myricetin, a naturally occurring flavonoid
Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the e...

Bok Yun Kang, Seung Hyun Kim, Daeho Cho, Tae Sung Kim in Archives of Pharmacal Research (2005)

13.Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a crucial mediator in response to inflammation. Myricetin protects cardiomyocytes against inflammatory injury. However, it’s still unexplored whe...

Jinliang Sun, Jianhui Sun, Xuezhong Zhou in European Journal of Medical Research (2019)

 

14.A DFT-based study of the hydrogen-bonding interactions between myricetin and ethanol/water
Flavonoids are vital constituents of propolis that are responsible for its medicinal activity. Flavonoid extraction commonly employs ethanol and water as solvents. In the extraction reaction, hydrogen-bonding int...

Yan-Zhen Zheng, Geng Deng, Rui Guo, Da-Fu Chen, Li-Ming Wu in Journal of Molecular Modeling (2019)

15.Validated HPTLC Method for Quantification of Myricetin in the Stem Bark of Myrica esculenta Buch. Ham. Ex D. Don, Myricaceae
A simple, rapid, and precise high-performance thin-layer chromatographic method has been established for quantitative analysis of myricetin in the stem bark of Myrica esculenta, family Myricaceae. The marker myricetin

Kalpana G. Patel, Vandana G. Patel… in JPC – Journal of Planar Chromatography – M… (2010)

 

16.Effects of myricetin, an anticancer compound, on the bioavailability and pharmacokinetics of tamoxifen and its main metabolite, 4-hydroxytamoxifen, in rats
This study examined the effect of myricetin, an anticancer compound, on the bioavailability ... 4-hydroxytamoxifen, in rats. The effect of myricetin on P-glycoprotein (P-gp), cytochrome ... (CYP)3A4 and 2C9 activ...

Cheng Li, Sung-Cil Lim, Jin Kim… in European Journal of Drug Metabolism and Ph… (2011)

17.Enhancement of recombinant myricetin on the radiosensitivity of lung cancer A549 and H1299 cells
Myricetin, a common dietary flavonoid is widely distributed ... this study was to investigate the effects of myricetin on combination with radiotherapy enhance radiosensitivity of...

Shijie Zhang, Lei Wang, Hongchun Liu, Guoqiang Zhao, Liang Ming in Diagnostic Pathology (2014)

18.Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS Myricetin, a naturally occurring flavonol, shows multifarious ... In order to obtain an understanding of the myricetin’s metabolism in vivo, a rapid and... n ...) techniques was ...

Yun Lin, Bin Wu, Zhixiong Li, Ting Hong, Mingcang Chen, Yingzi Tan… in Chromatographia (2012)

 

19.Thermochemistry of myricetin flavonoid The enthalpies of myricetin dissolution are measured by means of calorimetry...

A. Z. Abil’daeva, Sh. B. Kasenova, B. K. Kasenov… in Russian Journal of Physical Chemistry A (2014)

20.The role of the glycosyl moiety of myricetin derivatives in anti-HIV-1 activity in vitro Plant extracts are sources of valuable compounds with biological activity, especially for the anti-proliferative activity against pathogens or tumor cells. Myricetin is a flavonoid found in several plants ... act...