HPLC法測定苦杏仁甙含量的操作規程

起草單位：長沙上禾生物科技有限公司

日期：

目  的：HPLC法測定苦杏仁甙含量的操作規程

責任人：QC員。

內  容：

1、 試劑

1.1乙腈（HPLC級）

1.2 蒸餾水（AR級）

1.3磷酸（HPLC級）

1.4 甲醇（HPLC級）

2、 對照品

苦杏仁甙（購自中國藥品生物制品檢定所）

3、 儀器和用具

3.1 電子天平（1/100000）

3.2 玻璃儀器：100ml容量瓶

3.3 安捷倫1220 HPLC

4、液相條件

4.1流動相：乙腈一0.1％磷酸溶液(8：92)

4.2檢測波長：207nm

4.3流速：1ml/min

4.4柱溫：30℃

5 、  操作步驟

5.1 對照品溶液的制備:取在110℃乾燥至恆重的苦杏仁苷對照品適量，精密稱定，加甲醇製成每1m1含40μlg的溶液，即得。

 5.2樣品溶液制備  精密稱取在110℃乾燥至恆重的苦杏仁甙，精密稱定，加甲醇製成每1m1含40μlg的溶液，即得。

6、檢測

取對照品溶液和樣品溶液0.22μm針孔過濾器過濾，進樣20µl，根據峰面積計算樣品含量。

含量按以下公式計算：

           A2 x C1 x T

   R=------------------------- x 100

       A1 x C2

C1: 對照品的參考濃度，mg/ml

C2: 樣品的參考濃度，mg/ml

A1: 對照品溶液的色譜主峰值面積

A2: 樣品溶液的色譜主峰值面積

T:  對照品的含量

參考文獻：

 

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Armeniacae Semen contains not only amygdalin but also emulsin, which is an enzyme that hydrolyzes amygdalin. This hydrolysis reaction has been a major...d-amygdalin, the extraction time was kept to less...d-amygdalin
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Plasma and urine concentrations of amygdalin, whole-blood concentrations of cyanide, and...2...) and oral (500-mg tablet) administration of amygdalin. To measure low plasma concentrations of amygdalin following o...

Matthew M. Ames, Thomas P. Moyer, John S. Kovach… in Cancer Chemotherapy and Pharmacology (1981)

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Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to ... solution was used as the e...

Woo-Sang Joo, Ji-Seon Jeong, Hyogeun Kim, Yong-Moon Lee… in Archives of Pharmacal Research (2006)

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A quantitative 1H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin

Rie Tanaka, Akane Nitta, Akito Nagatsu in Journal of Natural Medicines (2014)

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Amygdalin is a cyanogenic glycoside occurring among others ... screening microbial cultures for their ability to degrade amygdalin is a convenient micro-titre version based...

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Ephedra alkaloids, including (EP), pseudo (PEP) and methyl (MEP), are sympathomimetic compounds with known toxicities but many Ephedra (Ephedrae herba...) preparations, such as Ephedra...

Yinghong Tang, Mengkai Zheng, Yu-Lin Chen… in European Journal of Drug Metabolism and Ph… (2017)

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A critical evaluation of a method for recovering HCN from cell extracts is presented. Since crude extracts often bind or metabolize HCN extensively, the HCN recovered by distillation at room temperature repres...

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In spite of several studies that have shown the cytotoxic effects of amygdalin on the different cancer cell lines, however, the chemopreventive potential of amygdalin on the breast cancer cell line is ... complet...

Bahman Moradipoodeh, Mostafa Jamalan, Majid Zeinali… in Molecular Biology Reports (2019)

26.Über ein neues, dem Amygdalin ähnliches Glucosid
Das Amygdalin, welches bekanntlich durch Emulsin im Sinne der... gespalten wird, ist schon vor 25 Jahren von Hrn. Hugo Schiff1) mit dem ...

Emil Fischer in Untersuchungen Über Kohlenhydrate und Fermente (1884–1908) (1909)

27.Sensitive Monitoring of Amygdalin and 5-Hydroxytryptamine in Food Supplements Using HILIC OH5 Chromatography
Amygdalin is a cyanogenic glycoside found in pits ... Recently, in some countries, food supplements containing amygdalin have been offered for the use in the alternative cancer treatment. The effectivity of amygdalin

Lucie Havlíková, Martina Parmová, Petr Chocholouš, Petr Solich in Food Analytical Methods (2016)

28.The anti-cancer effect of amygdalin on human cancer cell lines
Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and ... In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two ...

Asghar Arshi, Sayed Mostafa Hosseini… in Molecular Biology Reports (2019)

29.The pharmacokinetics of amygdalin
Amygdalin (d-mandelonitrile-β-d...-gentiobioside) is a cyanogenic glycoside claimed to show anti-cancer activity, sold under the incorrect name “Laetrile”. For a sensible discussion of its alleged activity and it...

A. G. Rauws, M. Olling, A. Timmerman in Archives of Toxicology (1982)

30.Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis
The aim of the present study was to evaluate the protective effects of combined atorvastatin and amygdalin in a rat model of endometriosis. Tumor ... suggested that combined treatment consisting of atorvastatin a...

Fang Hu, Yichuan Hu, Fangxin Peng in AMB Express (2019)