HPLC法测定苦杏仁甙含量的操作规程

起草单位：长沙上禾生物科技有限公司

日期：

目  的：HPLC法测定苦杏仁甙含量的操作规程

责任人：QC员。

内  容：

1、 试剂

1.1乙腈（HPLC级）

1.2 蒸馏水（AR级）

1.3磷酸（HPLC级）

1.4 甲醇（HPLC级）

2、 对照品

苦杏仁甙（购自中国药品生物制品检定所）

3、 仪器和用具

3.1 电子天平（1/100000）

3.2 玻璃仪器：100ml容量瓶

3.3 安捷伦1220 HPLC

4、液相条件

4.1流动相：乙腈一0.1％磷酸溶液(8：92)

4.2检测波长：207nm

4.3流速：1ml/min

4.4柱温：30℃

5 、  操作步骤

5.1 对照品溶液的制备:取在110℃干燥至恒重的苦杏仁苷对照品适量，精密称定，加甲醇制成每1m1含40μlg的溶液，即得。

 5.2样品溶液制备  精密称取在110℃干燥至恒重的苦杏仁甙，精密称定，加甲醇制成每1m1含40μlg的溶液，即得。

6、检测

取对照品溶液和样品溶液0.22μm针孔过滤器过滤，进样20µl，根据峰面积计算样品含量。

含量按以下公式计算：

           A2 x C1 x T

   R=------------------------- x 100

       A1 x C2

C1: 对照品的参考浓度，mg/ml

C2: 样品的参考浓度，mg/ml

A1: 对照品溶液的色谱主峰值面积

A2: 样品溶液的色谱主峰值面积

T:  对照品的含量

参考文献：

 

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Armeniacae Semen contains not only amygdalin but also emulsin, which is an enzyme that hydrolyzes amygdalin. This hydrolysis reaction has been a major...d-amygdalin, the extraction time was kept to less...d-amygdalin
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Plasma and urine concentrations of amygdalin, whole-blood concentrations of cyanide, and...2...) and oral (500-mg tablet) administration of amygdalin. To measure low plasma concentrations of amygdalin following o...

Matthew M. Ames, Thomas P. Moyer, John S. Kovach… in Cancer Chemotherapy and Pharmacology (1981)

20.Prevention of epimerization and quantitative determination of amygdalin in Armeniacae Semen with Schizandrae Fructus solution
Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to ... solution was used as the e...

Woo-Sang Joo, Ji-Seon Jeong, Hyogeun Kim, Yong-Moon Lee… in Archives of Pharmacal Research (2006)

21.Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus
A quantitative 1H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin

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Amygdalin is a cyanogenic glycoside occurring among others ... screening microbial cultures for their ability to degrade amygdalin is a convenient micro-titre version based...

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Ephedra alkaloids, including (EP), pseudo (PEP) and methyl (MEP), are sympathomimetic compounds with known toxicities but many Ephedra (Ephedrae herba...) preparations, such as Ephedra...

Yinghong Tang, Mengkai Zheng, Yu-Lin Chen… in European Journal of Drug Metabolism and Ph… (2017)

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A critical evaluation of a method for recovering HCN from cell extracts is presented. Since crude extracts often bind or metabolize HCN extensively, the HCN recovered by distillation at room temperature repres...

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In spite of several studies that have shown the cytotoxic effects of amygdalin on the different cancer cell lines, however, the chemopreventive potential of amygdalin on the breast cancer cell line is ... complet...

Bahman Moradipoodeh, Mostafa Jamalan, Majid Zeinali… in Molecular Biology Reports (2019)

26.Über ein neues, dem Amygdalin ähnliches Glucosid
Das Amygdalin, welches bekanntlich durch Emulsin im Sinne der... gespalten wird, ist schon vor 25 Jahren von Hrn. Hugo Schiff1) mit dem ...

Emil Fischer in Untersuchungen Über Kohlenhydrate und Fermente (1884–1908) (1909)

27.Sensitive Monitoring of Amygdalin and 5-Hydroxytryptamine in Food Supplements Using HILIC OH5 Chromatography
Amygdalin is a cyanogenic glycoside found in pits ... Recently, in some countries, food supplements containing amygdalin have been offered for the use in the alternative cancer treatment. The effectivity of amygdalin

Lucie Havlíková, Martina Parmová, Petr Chocholouš, Petr Solich in Food Analytical Methods (2016)

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Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and ... In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two ...

Asghar Arshi, Sayed Mostafa Hosseini… in Molecular Biology Reports (2019)

29.The pharmacokinetics of amygdalin
Amygdalin (d-mandelonitrile-β-d...-gentiobioside) is a cyanogenic glycoside claimed to show anti-cancer activity, sold under the incorrect name “Laetrile”. For a sensible discussion of its alleged activity and it...

A. G. Rauws, M. Olling, A. Timmerman in Archives of Toxicology (1982)

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The aim of the present study was to evaluate the protective effects of combined atorvastatin and amygdalin in a rat model of endometriosis. Tumor ... suggested that combined treatment consisting of atorvastatin a...

Fang Hu, Yichuan Hu, Fangxin Peng in AMB Express (2019)