About Macleaya cordata extract

Macleaya cordata is a bloodroot which owns thousand year history of medicinal usage in China. 13 kinds of alkaloids and other compounds such as sanguinarine toddaline, protopine, a-allocryptopine, bocconine, rhizoma coptidis alkali,berberine, samin alkali and protopine are in the root and aerial parts.

 Macleaya cordata extract contains sanguinaine and chelerythrine, which have fungicidal and insecticidal activity. Sanguinarine (pseudochelerythrine) is a potent inhibitor of NF-kappaB activation, IkappaBalpha phosphorylation, and degradation.

 Macleaya cordata contains several alkaloids including sanguinarine, chelerythrine,protopine, and allocryptopine. Our product, Macleaya cordata extract mainly constains with sanguinarine and chelerythrine. It was been show to be reversible inhibitors of the enzymatic hydrolysis of acetylthiocholine.

 

Macleaya cordata extract
Active Ingredients	Specs	Test Method	Appearance	Solubility
Total alkaloids	60%	HPLC	Orange yellow powder	Soluble in water
Sanguinarine	40%	HPLC	Orange yellow powder
chelerythrine	20%	HPLC	Orange yellow powder

Macleaya cordata extract Function:

1. The microbial disease,anti-inflammatory effects;
2. Insecticidal role;
3. Kill maggots;
4. Improve liver function and enhance immunity;
5. Can be widely applied to feed additives, antibacterial agent, pesticides, veterinary drugs, disinfectants, etc.

 

Macleaya cordata extract Application:

1. Applied in Food field;

2. Applied in Pharmaceutical field;

3. Applied in Feed additives field.

 

        Macleaya cordata extract HPLC

 

1.HPLC condition

Column：Hyper ODS C18

Detector: 270nm

Mobile Phase: AcCN:H2O =40:60

Flow Rate:1ml/min

Column length:200mm

Column diameter: 4.6mm

Pressure: 8.5 Mpa

Column temperature: room temperature

Injection volume: 5μl

 

2. Procedure

Standard preparation /calibration

  Accurately weigh 5mg Sanguinarine Reference Substance solution into 50ml Brown bottle. Add mobile phase to the scale, shake it well. Take 2ml of precise measurement and put it in 25 ml brown bottle, add methanol to the scale,shake it well, then abtain a susbstance solution.

 

Samples preparation

 Accurately weigh appropriate amount of Macleaya cordata extract sample, into 50 ml Brown volumetric flask,Add 30ml mobile phase and Ultrasound oscillation for about 30 minutes.Static to room temperature compatibility to mobile phase,filter through a 0.45μm PTFE filter then get the obtain a sample solution.

 

3. Determination method

Precise absorption respectively of reference solution and sample solution for 5ml each,Injection liquid chromatography and determination it ,and then it can be obtained.

 

Recommend Dosage

Macleaya cordate extraction process

Macleaya cordata fruit pod - - select and remove impurities - - - drying - - - heat to extraction (adjust PH to acidity) - - adjust PH to alkalinity - - - Filtering - - - filtering residue and ethanol dissolution - - - concentration - - - Filtering- - - drying - - - crush

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