Vine tea extract dihydromyricetin

Source:Vine tea

Latin name:Ampelopsis grossedentata(Hand-Mazz)WT Wan

Extract part:Leaf

Specifications: 5%-98%
Active Ingredient :Dihydromyricetin


Test Method:HPLC
Appearance: Fine white powder 

Vine tea extract introduction:

The vine tea tree is a rare and typical tea tree, known as dragon boat tea, dragon whisker tea, growing in hillside shrubs or valley woodland of 1300-1950 meters, has been regarded as the legendary health tea. Studies have shown that vine tea has the effect of reducing inflammation, relieving cough, and also has a strong inhibitory effect on common bacteria in food.


Dihydromyricetin introduction

Dihydromyricetin (DHM) is a natural extract of vine tea. For centuries, DHM has been used in traditional Chinese medicine to treat hangovers and relieve alcohol.
Flavonoids such as staphylococcus and dihydromyricetin in vine tea are the main active components, and their MIC and MBC are basically the same as berberine, and the free radical scavenging activity is between 73.3 and 91.5%. It can also reduce oxidative damage in the body, delay aging, and reduce liver deformation and tissue necrosis.


How does Dihydromyricetin powder work?

There is no other product more proven to reduce hangover symptoms except Dihydromyricetin powder. It has been studied extensively on mice and interacts with alcohol in a very unique way.


First, Dihydromyricetin powder is able to actually dampen the effect alcohol has on your brain. It does this by binding to GABA receptors. GABA is a neurotransmitter effected by alcohol. After alcohol enters your blood stream and reaches your brain it binds to GABA receptors and enhances the effect you get from the GABA neurotransmitter. This leads to your heart rate slowing, feelings of relaxation, and eventually drunkenness. Dihydromyricetin powder also binds to GABA receptors and softens the effect alcohol has on them. This lessens acute alcohol withdrawal the next day which reduces hangover symptoms.


Second, Dihydromyricetin powder enhances your livers ability to metabolize alcohol and alcohol’s toxic by product, acetaldehyde. When alcohol is metabolized in your liver it turns to acetaldehyde. Acetaldehyde is much more toxic than alcohol and can cause extreme nausea, headaches, and sensitivity to light. Dihydromyricetin powder helps your body remove acetaldehyde more quickly which decreases hangover symptoms the next day.


Dihydromyricetin Function

1.Dihydromyricetin has the best effect of anti-alcohol;

 2.Vine tea extract can protect the liver and stomach;

 3.Dihydromyricetin has the best effect of anti-hypertension;antitumor, antioxidation,anti-inflammation;

4.Vine tea extract has the best effect of antibiotic action;

5.Dihydromyricetin has the best effect of cardiovascular 

protection and  enhancing immunity.


Dihydromyricetin Application

1.Applied in food industry

2.Applied in health product, vine tea extract dihydromyricetin powder used as raw materials to enhance  body immunity

3.Applied in pharmaceutical field, vine tea extract dihydromyricetin powder used as capsules or pills to treat  respiratory tract infection and protect liver

                                                         Dihydromyricetin by HPLC



1.1     Methanol (AR)

1.2     Double distilled water (HPLC)

1.3     Reference substance 

1.4     Acetic Acid  (HPLC)



2.1     Electronic balance (1/100000)

2.2     Glass instrument: volumetric flask

2.3     0.45μm microfiltration membrane and Syringe filter

2.4     Ultrasonic cleaning instrument

2.5     HPLC

2.5.1       Agilent 1220 infusion pump

2.5.2       Agilent 1220 SPD-20A UV-VIS Detector

2.5.3       Agilent 1220 workstation


3. Chromatographic condition

3.1     Chromatographic column: LUNA ODS2 , 250mm×4.6mm, 5µ

3.2     Mobile phase: Methanol: Water: Acetic Acid =50:50:1 (v/v)

3.3     Flow rate: 1.0ml/min

3.4     Detection wavelength:292nm

3.5     Sample size: 20μl

3.6     Column temperature: room temperature


4 .Operate step

4.1     preparation of test sample solution

4.1.1       Precision weigh 1mg sample, put in the 10ml volumetric flask

4.1.2       Dissolved with 50% of Methanol , ultrasound 15min,cool to room temperature, constant volume with 50% of methanol, shake up, Filtered with  0.45μm microfiltration membrane, stand-by.

4.1.3       Preparation of standard solution

Precision weigh appropriate of Dihydromyricetin, make up the solution concentration of 0.1mg/ml with 50% of methanol.

4.2     Inject 5μl test sample solution and standard solution each to HPLC.



Dihydromyricetin (%)=

        A1 x C2 x V

  R=-----------------x 100

   A2 x M1

 A1:total peak area of Dihydromyricetin

A2:peak area of standard substance

C2: concentration of standard substance (mg/ml)

M1: sample size (mg)

V: constant volume of sample



                                                 Dihydromyricetin working process

  Raw Material (Vine tea)-----------Choose and wish and wipe off impurity----------Crushing-Ethanol (Food Grade) 

 extraction---------Concentrate---------- centrifugal------------on macroporous resin column----------Respectively elution with 20%, 40%, 60%,80% ethanol-----------fractional collections---------decolorizing----------Crystallization---------More time Crystallization------------Drying---------Mixing, Screening, Packaging------------Storage



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