Application:

 

1. Eliminate free radical, antioxidation;

 

2. It has pharmacological action of anti-thrombus, anti-tumor, cardiac-cerebral vessels protection, dephlogisticate and antibiosis;

 

3. Relief alcoholic poisoning, liver protection etc;

 

4. Heat-clearing and detoxicating, eliminating rheumatism, bones and muscles enhancer;

 

5. Myricetin have the action of astringent, stimulant and emetic.

 

Function:

 

1.Reducing the levels of blood sugar:

Bayberry bark extract Myricetin has a good effect of reducing the levels of blood sugar.

 

 2.Anti-tumor:

Bayberry bark extract Myricetin has effective restrain to cell proliferation of some tumor cells.

 

3.Affecting the activation and proliferation of lymphocyte: 

Bayberry bark extract Myricetin can restrain the expression of CD69 which is the early activatory sign of T cells.

 

4.Low the neurotoxicity: 

Bayberry bark extract Myricetin can protect the neuron by restraining the neurotoxicity caused by Glutamic acid. Then it can hold neurotrosis effectively.

 

5.Antioxidation: 

Bayberry bark extract Myricetin Play a vital role on many neurologic disease such as ischaemia and Alzheimer’s disease through oxidative stress. It can reduce production and toxicity of the β-amylase.

 

6.Antagonism of platelet activiation factor(PAF): 

Bayberry bark extract Myricetin can restrain the gathering of WRP and release of 52HT caused by PAF. It can also restrain the rising of Serum ionized calcium in the platelet caused by PAF.

 

7.Protecting the liver:  

Bayberry bark extract Myricetin has the strong inhibitory action of the rise of ALT and AST in the blood Serum. It can low the total bilirubin in the blood serum. So myricetin has the strong action of lowing the aminotransferase and jaundice.

 MOA -Myrecetin

 

1、Chromatographic conditions

 

Chromatographic column：Kromasil -C₁₈ 250cm×4.6mm 5μm Mobile phase: methanol: 0.03%H3PO4 Water (50∶50)

Column temperature：30℃

 

Flow rate：0.7.0mL/min

 

Detection wavelength：290nm

 

 

Injection volume：10μL

 

2、Solution preparation

1. Preparation of reference solution Precision weighed dry to constant weight reference substance about 5mg in a 25 mL volumetric flask，Dissolve with methanol。

 

2. Preparation of Sample Solution accurately weighed extract samples about 80mg in 50mL volumetric flask add 30mL methanol，Ultrasonic oscillation 20min， Placed to room temperature，with Methanol to volume，filtered through a 0.45μm mmicroporous membrane，then get the obtain a sample solution。

 

3、DETERMINATION OF SAMPLES

In the chromatographic conditions, injection determination after stable instrument, baseline is stable

 

 

4.Result          

                          A × W_s × S       ×100    

         Assay%=       A_s × W     

      ----As     Reference substance peak area

     ----A     Total peak area in the sample of myricetin

     ---W_s    Sample volume of Reference sample（mg）

     ---W    Sample weight of the sample（mg）

      ---S     Reference substance purity

 

References:

1.A. Umarov, V.S. Batyk, A.M. Khaleckii, Chem. Nat. Comp. 7, 499 (1971)

2.V.A. Soboleva, R.K. Chagovets, Chem. Nat. Comp. 7, 509 (1971)

3.R.K. Chagovets, I.M. Persev, V.O. Soboleva, Farm. Zh. 22, 64 (1967)

4.O.M. Bondarenko, R.K. Chagovets, V.I. Litvinenko, G.V. Obolensova, B.I. Sila, T.B. Kigel, Farm. Zh. 26, 46 (1971)

5.K. Miller, R. Pohl, Planta Med. 18, 114 (1970)

6.V.A. Soboleva, R.K. Chagovets, O.I. Gladkaya, //In proceeding: Tez 2-go simpoziuma po fenolnim soedineniyam., (Alma-Ata, 1970), p. 148

 7.Effects of myricetin on the bioavailability of doxorubicin for oral drug delivery in Rats: Possible role of CYP3A4 and P-glycoprotein inhibition by myricetin
The purpose of this study was to investigate the effect of oral myricetin on the bioavailability and pharmacokinetics of orally ... rats for oral delivery. The effect of myricetin on the P-glycoprotein (P-gp) and...

Sang-Joon Choi, Sang-Chul Shin, Jun-Shik Choi in Archives of Pharmacal Research (2011)

8.A glycoside of myricetin from the flowers of Hibiscus cannabinusZ. P. Pakudina, B. Maksudova in Chemistry of Natural Compounds (1976)

Für die elektrophoretische Auftrennung und densitometrische Bestimmung der Flavonoide Kämpferol (1), Quercetin (2), Quercetagerol (3), Myricetin (4) und der Heteroside Robinosid (5), Quercitrosid (6), Isoquercitrosid (7), Rutosid (8) und Myricitrosid (9)
K. Söllner in Fresenius' Zeitschrift für analytische Chemie (1960)

9.Evaluation of the antioxidant potential of myricetin 3-O-α-L-rhamnopyranoside and myricetin 4′-O-α-L-rhamnopyranoside through a computational study
In this work, we present a computational study on the antioxidant potential of myricetin 3-O-α-L-rhamnopyranoside (Compound M3) and myricetin 4′-O-α-L-rhamnopyranoside (Compound M4′). Structural parameters, bond ...
Rodrigo A. Mendes, Shawan K. C. Almeida, Iuri N. Soares… in Journal of Molecular Modeling (2019)

10.Spektrophotometrische und fluorometrische Bestimmung von Myricetin (3,3′,4′,5,5′,7-Hexahydroxy-Flavon)
Myricetin (3,3′,4′,5,5′,...−4% Myricetin lassen sich mit einem relativen Fehler von...
Antonia S. Zaprjanowa in Microchimica Acta (1976)

11.Analgesic activity of myricetin isolated from Myrica rubra Sieb. et Zucc. leaves
Myrica rubra Sieb. et Zucc....leaves are commonly used as an astringent, antidiarrheic, and analgesics in folk medicine in China. In the present study, the analgesic activity of myricetin

Yan Tong, Xiao-Mian Zhou, Shu-Jun Wang, Yang Yang… in Archives of Pharmacal Research (2009)

12.Inhibition of interleukin-12 production in mouse macrophagesvia decreased nuclear factor-κB DNA binding activity by myricetin, a naturally occurring flavonoid
Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the e...

Bok Yun Kang, Seung Hyun Kim, Daeho Cho, Tae Sung Kim in Archives of Pharmacal Research (2005)

13.Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a crucial mediator in response to inflammation. Myricetin protects cardiomyocytes against inflammatory injury. However, it’s still unexplored whe...

Jinliang Sun, Jianhui Sun, Xuezhong Zhou in European Journal of Medical Research (2019)

 

14.A DFT-based study of the hydrogen-bonding interactions between myricetin and ethanol/water
Flavonoids are vital constituents of propolis that are responsible for its medicinal activity. Flavonoid extraction commonly employs ethanol and water as solvents. In the extraction reaction, hydrogen-bonding int...

Yan-Zhen Zheng, Geng Deng, Rui Guo, Da-Fu Chen, Li-Ming Wu in Journal of Molecular Modeling (2019)

15.Validated HPTLC Method for Quantification of Myricetin in the Stem Bark of Myrica esculenta Buch. Ham. Ex D. Don, Myricaceae
A simple, rapid, and precise high-performance thin-layer chromatographic method has been established for quantitative analysis of myricetin in the stem bark of Myrica esculenta, family Myricaceae. The marker myricetin

Kalpana G. Patel, Vandana G. Patel… in JPC – Journal of Planar Chromatography – M… (2010)

 

16.Effects of myricetin, an anticancer compound, on the bioavailability and pharmacokinetics of tamoxifen and its main metabolite, 4-hydroxytamoxifen, in rats
This study examined the effect of myricetin, an anticancer compound, on the bioavailability ... 4-hydroxytamoxifen, in rats. The effect of myricetin on P-glycoprotein (P-gp), cytochrome ... (CYP)3A4 and 2C9 activ...

Cheng Li, Sung-Cil Lim, Jin Kim… in European Journal of Drug Metabolism and Ph… (2011)

17.Enhancement of recombinant myricetin on the radiosensitivity of lung cancer A549 and H1299 cells
Myricetin, a common dietary flavonoid is widely distributed ... this study was to investigate the effects of myricetin on combination with radiotherapy enhance radiosensitivity of...

Shijie Zhang, Lei Wang, Hongchun Liu, Guoqiang Zhao, Liang Ming in Diagnostic Pathology (2014)

18.Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS Myricetin, a naturally occurring flavonol, shows multifarious ... In order to obtain an understanding of the myricetin’s metabolism in vivo, a rapid and... n ...) techniques was ...

Yun Lin, Bin Wu, Zhixiong Li, Ting Hong, Mingcang Chen, Yingzi Tan… in Chromatographia (2012)

 

19.Thermochemistry of myricetin flavonoid The enthalpies of myricetin dissolution are measured by means of calorimetry...

A. Z. Abil’daeva, Sh. B. Kasenova, B. K. Kasenov… in Russian Journal of Physical Chemistry A (2014)

20.The role of the glycosyl moiety of myricetin derivatives in anti-HIV-1 activity in vitro Plant extracts are sources of valuable compounds with biological activity, especially for the anti-proliferative activity against pathogens or tumor cells. Myricetin is a flavonoid found in several plants ... act...

Joseph T. Ortega, Alirica I. Suárez, Maria L. Serrano… in AIDS Research and Therapy (2017)

21.Anti-inflammatory activity of myricetin-3-O-β-D-glucuronide and related compounds Objective and Design: The anti-inflammatory effect of myricetinglucuronide (MGL) was investigated and structurally-related compounds were compared to examine the structure/activity-relationship ...

A. Hiermann, H. W. Schramm, S. Laufer in Inflammation Research (1998)

22.Chemical modification of the natural flavonoid myricetin A review is presented of our own results on the synthesis of nitro-, amino-, azo-, and bromo- derivatives of myricetin, as a result which 19 new derivatives...

V. V. Polyakov in Chemistry of Natural Compounds (1999)

23.Antioxidant properties of myricetin and quercetin in oil and emulsions
The effect of quercetin and myricetin on the stability of sunflower oil and ... , conjugated dienes, and headspace volatile analysis. Myricetin showed strong antioxidant activity in oils stored ... tocopherols an...

Andrea Roedig-Penman, Michael H. Gordon in Journal of the American Oil Chemists' Society (1998)

24.Integrative analysis of miRNA and mRNA profiles in response to myricetin in human endothelial cells
MicroRNAs (miRNAs) are associated with a diverse range of biological processes, human diseases, and metabolic disorders. Myricetin, which is the most abundant polyphenol class ... . The present study aimed to eva...

Seung Eun Lee, Gun Woo Son, Hye Rim Park, Young-Ho Jin, Cheung-Seog Park… in BioChip Journal (2015)