Fisetin

Smoke tree extract Fisetin

Product Name: Smoke tree extract
Form: Powder
Appearance : Yellow Fine Powder
Part Used : Leaf

Active ingredient: Fisetin

Cas No.：528-48-3

Specification: 10%,20%,50%,98%

Test Method : HPLC

About fisetin:

Smoke Tree Extract Fisetin is a flavonol, a structurally distinct chemical substance that belongs to the flavonoid group of polyphenols. It can be found in many plants, where it serves as a colouring agent. Its chemical formula was first described by Austrian chemist Josef Herzig in 1891. Fisetin can be found in various plants such as Acacia greggii, Acacia berlandieri,in the yellow dye young fustic from Rhus cotinus (Eurasian smoketree), in Butea frondosa (parrot tree), Gleditschia triacanthos, Quebracho colorado and the genus Rhus and in Callitropsis nootkatensis (yellow cypresses). It is also reported in mangoes.

Staherb Fisetin main function:

1. Fisetin can be used to cure rheumatism, dysentery, gastrological, hernia, abdominal distension, toothache, traumatic injures, and skin ulcer in clinical.
2. Fisetin can anti-inflammatory.
3. Fisetin can antiproliferative.
4. Fisetin can indications Rheumatoid arthritis, diarrhea, stomach pain, hernia pain,

abdominal distention, pain, bruises, swelling, sores, carbuncles.
5. Fisetin can dispeling wind and dehumidify.

Staherb Fisetin Application:

1. Applied in food field, Fisetin powder is a kind of nourishing food which has much benefits to brain;

2. Applied in pharmaceutical field, Fisetin powder can be used to treat coronary heart disease and has better effect;

3. Applied in cosmetic field, Fisetin powder owns the function of whitening, dispelling spot, anti-wrinkle, and activating skin cells.

For more prouct information pls contact email sales09@staherb.cn

Physical index:

analysis	Specification	Result	Test method
Physical Description
Appearance	Yellow powder	Complies	Visual
Odor	Characteristic	Characteristic	Organoleptic
Sieve Analysis	100% pass 80 mesh	Complies	80 mesh
Chemical Tests
Assay ( Fisetin )	98.0% Min	98.26%	HPLC
Loss on drying	5.0% Max	2.33%	5g/105℃/2hrs
Bulk Density	45-60g/100mL	Complies	/
Microbiology Control
Total plate count	1,000cfu/g Max	Complies	AOAC
Yeast & Mold	100cfu/g Max	Complies	AOAC
E. Coli	Negative	Negative	AOAC
Salmonella	Negative	Negative	AOAC
Conclusion	Complies with the standards.
General Status	Non-GMO, Non-irradiation, ISO & Kosher Certificated.
Packing and Storage
Packing: Pack in paper-carton and two plastic-bags inside.
Shelf life: 2 year when properly stored.
Storage: Store in a well-closed place with constant low temperature and no direct sun light.

Determination of Fisetin

Determination of Fisetin in Smoke Tree P.E. by HPLC

HPLC Conditions:

Column: Inertsil ODS-3 4.6×150mm 5μm

Flow rate: 1.0 mL/min

Detection wavelength: 368 nm

Mobile phase: Acetomitrile: water: phosphoric acid (23:77: 0.1, V/V)

Sensitivity: 0.01AUFS

Column Temperature: 25 ℃

Injection Volume: 10μl

Solution Preparation

1. Standard Preparation:

Accurately weigh approximately 3mg of Fisetin Standard into a 25ml volumetric flask. Dilute to volume with methanol.

2. Sample Preparation:

Accurately weigh approximately 10mg of Smoke Tree P.E. and transfer into a 50ml volumetric flask. Add a 30ml methanol and sonicate for 30min. After cool at room temperature and dilute to volume with methanol. Filter through a 0.45μm filter into an HPLC vial or centrifuge to obtain a clear test solution.

III. Calculation

Under the above chromatographic conditions, when the instrument is stable, and the sample was measured at baseline.

The retention time of Fisetin is about 20 min. The final results are quantified against external standard.

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