Bitter Melon Momordicoside by HPLC
Determination of Bitter Melon Momordicoside by HPLC
1) Reagents
a. Methanol (HPLC) b. Acetronitrile(HPLC) c. Nanopure water
2) Apparatus
HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner
0.45μm PTFE filter 50ml volumetric flask Screw cap vials
3) HPLC condition
Column: Kromasil ODS C18 5μm , 4.6×150mm
Detector: UV208nm
Mobile Phase: A=Water B= MeOH:Aceton=1:1
Flow Rate: 0.8ml/min
Injection: 10μl
4)Procedure
Samples Preparation
Accurately weigh 120mg extraction into 50ml volumetric flask. Add Methanol to volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial and proceed with the analysis.
Reference preparation /calibration
Prepare an reference solution (approximately 120mg/50ml in MeOH) using a commercial reference of Bitter Melon Momordicoside，Sonicate for about 10 minutes and transfer to screw-cap vials and can be stored in the refrigeration.
5) Calculation
Compare the peak areas of the Bitter Melon Momordicoside on the sample chromatogram and the corresponding peaks in the chromatogram for the reference solution.
1) Reagents
a. Methanol (HPLC) b. Acetronitrile(HPLC) c. Nanopure water
2) Apparatus
HPLC (Shimadzu) Analytical balance (1/10000) Ultrasonic cleaner
0.45μm PTFE filter 50ml volumetric flask Screw cap vials
3) HPLC condition
Column: Kromasil ODS C18 5μm , 4.6×150mm
Detector: UV208nm
Mobile Phase: A=Water B= MeOH:Aceton=1:1
Flow Rate: 0.8ml/min
Injection: 10μl
4)Procedure
Samples Preparation
Accurately weigh 120mg extraction into 50ml volumetric flask. Add Methanol to volume and sonicate to mix, filter through a 0.45μm PTFE filter into a screw cap vial and proceed with the analysis.
Reference preparation /calibration
Prepare an reference solution (approximately 120mg/50ml in MeOH) using a commercial reference of Bitter Melon Momordicoside，Sonicate for about 10 minutes and transfer to screw-cap vials and can be stored in the refrigeration.
5) Calculation
Compare the peak areas of the Bitter Melon Momordicoside on the sample chromatogram and the corresponding peaks in the chromatogram for the reference solution.